Part:BBa_K4947028:Design

M. sativa Chalcone Isomerase 1 Codon-Optimized CDS

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 627
  Illegal AgeI site found at 420
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The codon optimization and domestication was done to improve recombinant expression in E. coli and enable restriction enzyme-based swapping of promoters and terminators, respectively.

Source

This gene homolog that encodes for CHI was chosen rationally after thorough literature review. CHI is an enzyme involved in the biosynthetic pathway of daidzein, converting isoliquiritigenin into liquiritigenin, both stable intermediates. The gene sequence was sourced from NCBI GenBank [1], and produced by Twist Bioscience.

References

1. https://www.ncbi.nlm.nih.gov/protein/AAB41524.1

2. View our contributions page (https://2023.igem.wiki/yale/contribution) for a spreadsheet of all our sources!

3. Li, J, et al. (2021, September 25). Diversion of metabolic flux towards 5-deoxy(iso)flavonoid production via enzyme self-assembly in escherichia coli. Metabolic Engineering Communications. https://www.sciencedirect.com/science/article/pii/S2214030121000250#bib64

4. Cross-kingdom expression of synthetic genetic elements promotes discovery of metabolites in the human microbiome. Patel JR, Oh J, Wang S, Crawford JM, Isaacs FJ. Cell. 2022 Apr 28;185(9):1487-1505.e14. doi: 10.1016/j.cell.2022.03.008. Epub 2022 Apr 1. 10.1016/j.cell.2022.03.008 PubMed 35366417

5. Yan, Yajun., et al. (2007). Biosynthesis of 5-deoxyflavanones in microorganisms. Wiley Online Library. Biotechnology JournalVolume 2, Issue 10: Multi-enzyme Systems for the Synthesis of Complex Natural Compounds. https://onlinelibrary.wiley.com/doi/epdf/10.1002/biot.200700119